Explain the need for CaCl2 in the in vitro assay system. b. How do pH and salt concentration influence mixed micelle formation? c. Suggest a method that could be used to monitor the enzymatic reactions continuously. d. Why would spectrophotometry be an unattractive method for following phospholipase reactions? e. Provide a reasonable explanation for the ability of phospholipase to distinguish between aggregated and molecularly dispersed phospholipids. f. Why do phospholipase Ae’s from different sources (bee venom vs. pork pancreas) have different specificities toward different PC / detergent micelles

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